重组白细胞介素2制品外源残余DNA的检测

Detection of Exogenous Residual DAN in Recombinant Interleukin 2 Products

采用ＤＮＡ杂交技术，用３２Ｐ标记的探针检测重组白细胞介素２制品的外源ＤＮＡ残余量。设三个水平：２５个剂量、１５个剂量、５个剂量。结果提示该方法是一种敏感有效的检测方法，其最小用样量为５个剂量单位。这为新制品检测方法的建立提供依据。

The technique of DNA hybridization was applied to detect exogenous residual DNA in rIL-2 productrs with 32 P as a probe. There were three levels:25 doses, 15 doses and 5 doses, in this test. The result showed the method was a sensitive and effective way, and the smallest doses of sample was 5 doses.It provides a basis for establishing method to detect a kind of new product