腰椎间盘内破裂模型的建立

AN EXPERIMENTAL MODEL OF LUMBAR INTERNAL DISC DISRUPTION

目的:建立腰椎间盘内破裂模型并行组织学和椎间盘造影CT观察。方法:15只山羊,手术损伤L5,6前部全层纤维环,L6,7后部纤维环内层,术后3周和3、6个月观察椎间盘造影CT的变化,并进行组织学检查,评定纤维环损伤后的病理改变。结果:组织学和椎间盘造影CT显示L4,5和L5,6在实验期间未发现有后部纤维环内破裂,L6,7后部纤维环内层破裂处未愈合,并在3、6个月时破裂向纤维环外部不断延伸,但均未穿破纤维环外层。结论:经山羊腰椎间盘前方人为损伤后部纤维环内层,可以可靠、一致地制备椎间盘内破裂动物模型。

Objective： To establish the experimental model of lumbar internal disc disruption and to observe the alteration of histology and CT/discography. Methods： 15 goats were subjected to surgery. L5.6 intervertebral discs were full-thickness ventral stab of annulus fibrosus, L6,7 intervertebral discs were stab of posterior internal annulus fibrosus. CT/discography and the histological examination were taken at the 3 weeks, 3 and 6 months after operation, in order to assess the degenerative and pathological change of intervertebral discs. Results： It was observed in histology and CT/discography that L4.5 and L5,6 intervertebral discs were not found the disruption of posterior internal annulus fibrosus. The disruption of L6,7 posterior internal annulus fibrosus unhealed, enlarged and spreaded to the exterior annulus at 3, 6 months after operation. Conclusion： Stabbing the posterior internal annulus fibrosus through goat anterior lumbar discs results in a reliable uniforml animal model of internal disc disruption.