摘要
悬铃木（Ｐｌａｔａｎｕｓ ｏｃｃｉｄｅｎｔａｌｉｓ Ｌ．）突变体是其种子经γ射线辐射培育出的一种营养生长正常而开花发育过程受阻的不育植株。经过１０多年的观察研究证明：突变体的花芽不能形成是因为突变体细胞染色体有部分缺失。应用基因组相减技术分离这些在突变体中缺失但在野生型中存在的ＤＮＡ，经过４次循环富集缺失片段后克隆了１个２．０ ｋｂ 的ＤＮＡ 片段，ＤＮＡ 杂交证明此片段只存在于野生型基因组中而不存在于突变体中。
A novel type of mutant of planetree (Platanus occidentalis L.), which was produced via γ ray irradiation, does not form flower buds yet its vegetative organs grow normally. Genetic analysis showed that this was due to the deletion of DNA fragments in the mutant genome. Through genomic subtraction technique, a 2.0 kb DNA segment (pBS 2.0 ) was isolated, which is only present in wild planetree genome, but is absent in mutant genome.
基金
中国科学技术大学青年基金
关键词
悬铃木
DNA
基因组相减
缺失突变体
Genomic subtraction
Deletion mutant
Platanus occidentalis
