摘要
采用聚丙烯酰胺凝胶垂直板电泳法，分析了Ｚｍｕ１：ＤＨＰ豚鼠心、肝、脾、肺、肾、骨骼肌、红细胞和血清的ＬＤＨ及ＳＯＤ同工酶，对ＬＤＨ同工酶谱作了光密度扫描，计算出各同工酶组分的相对活力及不同组织和血清ＬＤＨ中Ａ、Ｂ亚基的含量。Ｚｍｕ１：ＤＨＰ豚鼠肝中缺乏ＤＨＰ豚鼠和其他实验动物肝中普遍存在的ＬＤＨ５组分，多数组织中ＬＤＨＢ亚基含量呈不同程度提高。Ｚｍｕ１：ＤＨＰ豚鼠各组织和血清的ＳＯＤ同工酶呈多带分布。ＳＯＤ１和ＳＯＤ２为共有酶带。
Lactate dehydrogenase and superoxide dismutase isoenzymes in tissues of the heart, liver,spleen,lung,kidney,skeletal muscle, red blood cell and serum of Zmu1:DHP guinea pig were analysed with PAGE. The results revealed that LDH 5 was absent in the liver and the contents of LDH B subunit in most tissues were increased in varying degrees, and the SOD from different tissues and serum were appeared in multiband distribution on the electrophoretic pattern. Both SOD 1,and SOD 2 were the common bands in the zymograms for all the sampled tissues.
出处
《上海实验动物科学》
1996年第3期145-148,共4页
Shanghai Laboratory Animal Science
