大肠杆菌原核增强子样M片段的进一步研究

A Further Study on the Enhancer-like M Fragment in Escherichia coli JM103 Genome

阐明基因转录调控机理一直是分子生物学的研究热点。增强子是广泛存在于真核、病毒及原核生物中的重要的调控元件之一,它通过与各种调控蛋白因子相互作用而发挥其转录增强调节功能。 原核转录增强子的发现是近几年的事,有关研究报道远少于真核和病毒增强子。1989年,潘卫。

With the enhancer detecting vector pKN2 a genomic SM fragment about 355bp long was found to have prokaryotic enhancer-like properties. The smaller fragment was obtained by cutting a larger M fragment with Bst E I . It can enhance the expression of CAT gene in its cognate host strains (E. coli) by 3-7 times.