戊四氮点燃过程中大鼠海马BMP4表达与神经增殖关系

Relation of BMP4 and neurogenesis of hippocampus formation in adult rats during pentylenetetrazol kindling

目的探讨戊四氮(PTZ)点燃过程中大鼠海马骨形成蛋白4(bone morphogenetic protein-4,BMP4)的表达变化与神经增殖的关系。方法将成年大鼠分为对照组与模型组,模型组大鼠又根据在点燃中的不同时相点分为9组。用免疫组织化学与原位杂交的方法检测海马齿状回BMP4mRNA与BrdU阳性细胞数的变化。结果正常成年大鼠BMP4阳性细胞主要分布于齿状回的门区、颗粒下层、CA3、CA1区。BrdU阳性细胞主要分布在齿状回颗粒下层。BMP4阳性细胞与BrdU阳性细胞在点燃过程中均明显增加,呈明显正相关,点燃后2月降至基线水平。结论BMP4可能通过影响成年大鼠海马神经发生在PTZ点燃过程中起重要作用。

Objective To investigate the relationship between the expression of bone morphogenetic protein- 4 （BMP4） in the hippocampus and the neurogenesis during the pentylenetrazol（PTZ） kindling process of the adult rats. Methods Rats were divided into two groups：the control group and the PTZ kindling group. And the PTZ kindling group includes 9 subgroup according to different time phase. The changes of the BrdU labelled cells and BMP4 mRNA-positive cells of the hippocampus and denate gyrus was observed by immunohistochemical and in situ hybridization methods. Results The BMP4 mRNA positive cells were mainly distributed in the hilus and subgran ular zone of dentate gyrus and CA3 and CA1 of the hippocampus of adult rats. The BrdU labelled cells were mainly distributed in the subgranular zone. The numbers of BMP4 mRNA-positive cells and BrdU labelled cells increased significantly during PTZ kindling process and decreased to base line 2 months later. There were positive correlation between the number of BMP4 mRNA-positive cells and the number of BrdU labelled cells. Conclusion BMP4 like ly plays an important role in the onset of seizures through adult neurogenesis of the hippocampus induced by pentylenetrazol.