武汉地区汉人2型糖尿病合并肥胖及非肥胖患者Lepr基因变异的对比性分析

Comparison of Lepr Gene Variant between Patients with Obesity and ones without Obesity in the Hans with Type 2 Diabetes Mellitus in Wuhan District

目的研究瘦素受体基因GIN223Arg与2型糖尿病合并肥胖的关系。方法运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定无亲缘关系且具有完整资料的武汉地区汉人351例(其中2型糖尿病合并肥胖组131例、2型糖尿病非肥胖组148例、正常对照组72例)。结果①2型糖尿病合并肥胖组基因型AA、AG、GG(分别为0.398、0.480和0.122)与2型糖尿病非肥胖组的(0.196、0.223和0.581)和正常对照组分别为(0.208、0.264和0.528)比较有显著性差异(P<0.01)。②2型糖尿病合并肥胖组等位基因分布频率A、G分别为0.642及0.358,与非肥胖组及正常对照组比较有统计学意义(P<0.01)。③应用多重回归分析(NominalRegression)2型糖尿病与临床各变量的关系发现,2型糖尿病GIN223Arg变异(由A突变G)与BMI、收缩压、舒张压(分别为P=0.001,P=0.032,P=0.037)相关。结论瘦素受体基因GIN223Arg变异可能与2型糖尿病合并肥胖和高血压相关。

Objective To investigate the relationship between Gln 223 Arg variant ,a leptin receptor gene and obesity accompanied with type 2 diabetes mellitus. Methods The genotypes of Gln223Arg variant were determined by PCR-RFLP in 351 unrelated subjects of Wuhan ＂Han＂ population who included 72 subjects with normal glucose tolerance as control, 131 with obesity in type 2 diabetic patients and148 without obesity in type 2 diabetic patients）. The clinical data were also analyzed. Results In patients with obesity in type 2 diabetic patients, the gcnotype frequency was AA, AG and GG（0.398,0480 and 0. 122 respectively）, while in patients without obesity in type 2 diabetic patients, the genotype frequency was AA, AG and GG（0. 196 , 0. 223 and 0. 581 respectively）, and in control it was AG, GG and AA（0. 208 . 0. 264and 0. 528 respectively）. There were significant differences in the genotype frequency of Gln223 Arg variant in leptin receptor gene among the three groups （ P 〈0. 001 ）. In the obesity in type 2 diabetic patients ＂A＂ and ＂G＂ allele were 0. 642 and 0. 358 respectively , which were significantly different from those without obesity in type 2 diabetic patients and control subjects （ P 〈0.01, P 〈0.001 ）. Logistic regression analysis showed that this gene variant was an independent risk factor of type 2 diabetic patients accompanied with obesity. Conclusion Gln 223 Arg variant in leptin receptor gene is associated with obesity and hypertension in type 2 diabetic patients