高效脱酚菌的筛选及微生物多样性分析

Screening and Microbial Diversity Analysis of High-Efficiency Phenol-Degradation Bacteria

为了揭示不同培养基分离的细菌种群的多样性差异及获得高效脱酚菌,采用单链构象多态性技术,以16S rRNA基因的V3区为靶对象,对3种不同培养基SJ(自制培养基)、LB(牛肉膏蛋白胨培养基)、YEB(酵母牛肉膏培养基)分离细菌的种群多样性进行了比较研究.结果表明:SJ培养基比LB和YEB培养基有更高的种群多样性,更适于细菌的分离筛选.利用SJ培养基筛选出的3株高效脱酚菌降解废水中总酚的能力均在99%以上,经过生理生化鉴定和16S rDNA测序,建立了系统发育树,鉴定出这3株菌分别属于气单胞菌属(Aeromonas)、芽孢杆菌属(Bacillus)和不动细菌属(Acinetobacter).

In order to reveal the differences in the microbial diversity of the bacilli stock cultured in different sub- strates as well as culture high-efficiency phenol-degradation bacteria, the V3 section during the 16S rRNA was used as the target to investigate and compare the biodiversities of bacteria isolates respectively cultured in three different isolation substrates, namely the self-prepared SJ substrate, the LB substrate and the YEB substrate. The results show that SJ substrate is more suitable for the isolation of bacteria with higher microbial diversity than LB and YEB substrates, and that the three strains of high-efficiency phenol-degradation bacteria screened by SJ substrate are all of a total-phenol removal rate of more than 99%. Moreover, according to the phylogenic tree established by means of physiological and biochemical appraisals and 16S rDNA sequencing, the three strains are respectively Aeromonas, Bacillus and Acinetobacter.