姜桂饮胶囊中桂皮醛及二苯基庚烷A含量测定

Assaying of cinnamaldehyde and diphenylheptane A in Jiangguiyin capsule

目的建立姜桂饮胶囊中桂皮醛和二苯基庚烷A含量测定方法。方法用Gc法测定桂皮醛含量，色谱柱为sE．30石英毛细管柱（30m×0．25mm×0．25μm），柱初始温度80℃保持1min，以2℃／min一阶升温至140oC，再以20oC／min二阶升温至200℃；用HPLC法测定二苯基庚烷A含量，选用C18柱，以甲醇-水（体积比63：37）为流动相，检测波长220nm。结果桂皮醛在2．0—20．0mg／mL质量浓度范围内，与桂皮醛及内标的峰面积比呈良好的线性关系，r=0．9994，平均回收率为98．2％；二苯基庚烷A在0．1104—1．104μg范围内，与峰面积呈良好线性关系，r=0．9996，平均回收率为99．4％。结论方法简便、准确、专属性强，可用于本品的质量控制。

Objective To establish the method for assaying the content of cinnamaldehyde and diphenylheptane A in Jiangguiyin capsules. Methods Cinnamaldehyde was analyzed on quartz capillary column （SE-30 30 m × 0.25 mm×0.25 μm）by GC. The initial temperature of the column was 80 ℃ for 1 min, increased to 140℃ with 2 ℃/min, then to 200℃ with 20 ℃/min. Diphenylheptane A was analyzed on an HPLC column （200 mm×4.6 mm, 5 μm） by using methanol： water （63： 37） as mobile phase. The detection wavelength was 220 nm. Results The calibration curves were linear over the range of 2.0 -20. 0 mg/mL （ r = 0. 9994 ） for cinnamaldehyde and 0. 1104 -1. 104 μg （ r = 0. 9996 ） for diphenylheptane A. The average recoveries of cinnamaldehyde and diphenylheptane A were 98.2% and 99.4%, respectively. Conclusion The method is reliable, accurate and specific, which can be used for the quality control of Jiangguiyin capsule.