不同产地川芎中丁烯基苯酞的HPLC分析

HPLC analysis of butylidenephthalide in Szechwan lovage rhizome from different habitats

目的建立川芎药材中丁烯基苯酞的含量测定方法。方法采用RP—HPLC法对不同产地川芎中丁烯基苯酞的含量进行测定。色谱柱：C18（200mm×4．6mm，5μm）；流动相：甲醇-水（体积比67：33）；流速：1．0mL／min；检测波长：230nm。结果丁烯基苯酞在6．96～69．60μg/mL范围内线性关系良好（r=0．9996），平均回收率为97．79％（RSD=1．7％）。结论本法简便准确，重现性好，可用作川芎药材的质量控制，为不同产地的川芎药材的收购及栽培等提供有效监控依据。

Objective To establish a method for the content determination of butylidenephthalide in Szechwan lovage rhizome. Methods Butylidenephthalide in Szechwan lovage rhizome from different habitats was analyzed with RP-HPLC. A Diamonsil C18 （200 mm× 4.6 mm, 5 μm） column was used to separate the sample by using methanol-water （67： 33） as the mobile phase, The flow rate was 1.0 ml/ min and the detection wavelength 230 nm. Results The calibration curve was linear over the concentration of 6.96 - 69.60 μg/ml for butylidenephthalide （r = 0.9996）. The average recovery of butylidenephthalide was 97.79 % and RSD 1.7%. Cconclusion This method is simple and reproducible, which can be used for the quality control of Szechwan lovage rhizome.