顶空气相色谱法测定尿中1,2-二氯乙烷

Determination of 1,2-dichloroethane in urine with static headspace gas chromatography

目的建立尿中1，2-二氯乙烷的顶空气相色谱测定方法。方法将样品置于密闭的顶空瓶内，采用SE-30色谱柱（2m×3mm），在85℃进行分离，氢火焰离子化检测器检测（150℃），气化室温度150℃；载气（氮气）流速30mL／min，空气流速300mL／min，氢气流速30mL／min，对尿中1，2-二氯乙烷进行测定。结果本方法最低检出浓度为0，52mg／L，1，2-二氟乙烷在0—20．0mg／L范围内有良好线性（r=0．9980），平均回收率为97．2％及94．1％，RSD值为5．18％及6．58％。结论本方法简便、快速、准确，能用于尿中1，2-二氯乙烷的测定。

Objective To study the assay method of 1,2-dichloroethane in urine with static headspace gas chromatography and the biomarker of 1, 2-dichloroethanc exposure. Methods： 1, 2-dichloroethane in urine was determined with static headspace gas chromatography with SE-30 column at 85~C and detected with flame ionization detector（FID）. Results： The minimum of determination concentration was 0.52mg/L. 1,2-Dichloroethane had a good linearity （ r =0. 9980） within 0 ~ 20.0mg/L. The average recovery was 97.2% and 94.1%, with the RSD of 5.18% and 6.58%. Conclusions： The method is simple, rapid and accurate and can be used in determination of 1,2-Dichloroethane in urine.