FHIT、PTEN蛋白在胃癌中的表达及意义

Expression and significance of FHIT and TPTEN in gastric carcinoma

目的:探讨脆性组氨酸三联体(FH IT)基因和张力蛋白同源、第10染色体丢失的磷酸酶基因蛋白(PTEN)在胃癌中的表达及其与临床病理因素的关系。方法:采用免疫组织化学SP法和图像分析技术检测了80例胃癌组织及癌旁组织中FH IT和PTEN基因蛋白的表达。结果:80例胃癌组织中FH IT和PTEN蛋白阳性表达率分别为45%和51.3%,在癌旁组织中分别均为100%,其差异均有统计学意义(P<0.05)。FH IT蛋白的表达与胃癌的分化程度、浆膜浸润、淋巴转移无关(P>0.05),而与肿瘤分期有关(P<0.05)。PTEN蛋白的表达与胃癌的分化程度、浆膜浸润、淋巴转移、肿瘤分期均有关(P<0.05)。结论:FH IT和PTEN基因蛋白缺失在胃癌发生发展起重要作用,二者可作为判断胃癌生物学行为和临床预后的参考指标。

Objective：To investigate the expression of fragile histidine triad （FHIT） and phosphatase and tensinhomologue deleted on chromosome ten（PTEN） in gastric carcinoma and their relationship to clinicopatholcgical factors. Methods：Immunohistochemical（S-P） and image analysis methods were used to detect the expression of FHIT and PTEN in the 80cases of gastric carcinoma and their corresponding paracancerous tissues. Results： The positive rate of expression of FHIT,PTEN in 80 gastric carcinoma tissue were 45% and 51.3%, the paracancerous tissues showed all positive 100% ; the differences were statistically significant（P 〈0.05）. FHIT expression had no correlation with the degree of differentiation, range of tumor in invasion, lymph node metastasis （P 〉 0.05 ）, but was related with staging （ P 〉 0.05 ）. PTEN expression was correlated with the differentiation, range of invasion, lymph node metastasis and staging （ P 〈 0.05 ）. Conclusion： Decrease or absence expression of FHIT and PTEN protein plays an important role in the genesis and development of gastric carcinoma; the two protein can be used to judge carcinomatous biological behaviors and prognosis.