三维培养条件下WB-F344细胞膜流动性及粘附分子表达变化

Effect of three-direction culture conditions on WB-F344 cells membrane fluidity and the variation of adhesive molecules expression

目的探讨三维培养条件下对WB-F344细胞膜流动性的影响和粘附分子表达的变化。方法通过荧光偏振法检测细胞膜流动性变化,并用半定量RT-PCR技术检测细胞粘附分子表达变化情况。结果与平面培养的细胞相比,三维培养的细胞膜荧光偏振度与生物膜粘度下降,说明细胞膜流动性增加。三维培养和平面培养下,各种粘附分子均可表达,而三维培养条件下纤粘连蛋白(Fn)和整合素-β1(integrin-β1)mRNA的表达明显上调,整合素-α5的表达也有所增加。结论三维培养可提高WB-F344细胞膜流动性从而维持细胞膜的功能,并上调粘附分子的表达。

Objective To explore the effects of the three - direction cells membrane fluidity and the variation of adhesive molecules expression. （3D） culture conditions on WB- F344 Methods The cells mem tbrane fluidity was detected by fluorescence polarization, and the expression of adhesive molecules were measured by reverse transcriptase- polymerase chain reaction （RT- PCR）. Results The results demonstrated that the polarization of membrane fluorescence and viscousness decreased in 3D conditions compared with monolayer cultured cells, that means the membrane fluidity increased. The detected mRNA expression of adhesive molecules by RT- PCR demonstrated that on 3D culture and monolayer culture, although various adhesive molecules were expressed, Fn and integrin-β1 up - regulated more apparently and integrin -α5 also elevated slightly on 3D culture. 3D culture conditions can improve the membrane fluidity so to maintain the cell membrane function mRNA of adhesive molecules. Conclusion The and up-regulated mRNA of adhesive molecules.