RP-HPLC法测定平消片中马钱子碱和士的宁的含量

Determination the Contents of Bruchine and Strychine in Pingxiao Tablet by RP-HPLC

目的采用反相高效液相色谱法(RP-HPLC)测定平消片中马钱子碱和士的宁的含量。方法采用lichrospher C18色谱柱(4.6mm×250mm,5μm);流动相为水-乙腈(60∶40),每1000mL中含磷酸二氢钾3.4g、十二烷基硫酸钠1.7g;流速为1.0mL/min;检测波长254nm;柱温25℃。结果马钱子碱在0.102～0.918μg、士的宁在0.208～1.872μg范围线性关系良好,相关系数均为0.9999;平均回收率分别为101.07%(RSD=2.26%)、99.29%(RSD=2.79%)。结论本方法操作可靠、精确,适用于平消片中马钱子碱和士的宁的含量测定。

Objective To establish the RP-HPLC method for determination of Bruchine and Strychine in Pingxiao Tablet. Methods The lichrospher C18 （4.6 min×250 mm, 5μm） was Used as the stationary phase. The Mobile phase consisted of water-acetordtrile （60 ： 40） with 1.7 g sodium dodecylsulphate and 3.4 g KH2PO4 in 1000 mL mixture. The Detecting wavelength was 254 nm and flow rate was 1.0 mL/min. The column temperature was 25 ℃. Result The linear ranage of Bruchine and Strychine were 0.102-0.918μg and 0.208-1.872 μg respectively. The recovery rates of bruchine and strychnine were 101.07% （RSD=2.26%） and 99.29% （RSD=2.79%） respectively. Conclusion This method is realiable and accurate, the method can be applied to determination the contents of Bruchine and Strychine in Pingxiao Tablet.