利用正交设计优选复方三花茶剂中水溶性成分的提取工艺

Optimization of the Extraction Procedures of Compound Three-Flower-Tea by Orthogonal Design

目的建立TLC-UV法测定复方三花茶剂中绿原酸含量的方法,并利用正交试验优选复方三花茶剂中水溶性成分的提取工艺。方法建立TLC-UV法测定复方三花茶剂中绿原酸含量的方法后,以绿原酸含量及浸膏得率的综合评分为指标,采用正交试验法对提取过程中的温度(A)、浸出时间(B)、加水量(C)、浸出次数(D)4个因素进行优选。结果提取工艺中影响三花茶剂中绿原酸含量的显著因素是A、C、D。结论以绿原酸含量和浸膏得率二者的综合评分为考察指标的最优提取工艺为A3B2C3D3。

Objective To optimize the extraction procedures of compound Three-Flower-Tea （CTFT） by orthogonal design. Methods After having established the methods of quantifying the chlorogenic acid by TLC-UV, the orthogonal design was used to select the best extraction procedures, using chlorogenic acid content and extract yield as the measuring index, through controlling four different factors, naming extraction temperature （A）, extraction time （B）, watervolume added （C） and extraction number of times （D）, and three levels at each factor. Result The effective factors which influenced the extraction procedures were A, C and D. Couclusion The optimum extraction procedures for CTFT should be 15-fold water volume, three-time decocting with 20 minutes per time at temperature of 100 ℃.