铅硒联合作用对原代培养海马神经元影响

Effect of lead and selenium on primary cultured hippocampal neurons.

目的观察铅、硒对原代培养的海马神经元生长及存活的影响,研究硒对铅的神经细胞生长抑制的拮抗作用。方法建立新生Wistar大鼠海马神经元原代无血清培养技术,通过PbCl2,Na2SeO3单独及联合作用,观察神经细胞的生长和存活情况。结果10-5mol/L铅明显抑制了原代培养的海马神经元突起生长,引起神经元存活率下降;单独硒(2×10-6mol/L)有明显促进海马神经元突起生长的作用,尤其在神经元突起生长早期;但对海马神经元存活率的影响不明显。铅和硒共同孵育时,培养早期硒能拮抗铅对神经元突起延伸的抑制,但随着培养时间延长,这种拮抗作用消失;从神经元存活率来看,在实验剂量下,未见硒能拮抗铅对神经元存活的抑制作用。结论铅具有抑制神经元生长和存活的毒性,本实验剂量下的硒在细胞培养早期有促进神经元突起生长和拮抗铅对神经元生长和存活的抑制作用。

Objective To study the effect of lead and selenium on the growth and survival of primary cultured hippocampal neurons. Methods The primary culture of hippocampal neurons of newborn Wistar rats with serum-free medium was established. After co-cultured with PbCl2 and Na2SeO3 respectively and simultaneously, the growth of neurites and survival of neurons were tested. Results Pb2^＋ （10^-5 mol/L）delayed the growth of neurons, shortened the length of neurites, and decreased the neurons＇ survival rate. Selenium（2 × 10^-6 mol/L） stimulated the growth of neurites, especially at early time of culture. There was no significant effect of selenium at studied dosage on survival rate of hippocampal neurons. The results of co-cultivated with PbCl2 and along with the culture time prolongation, the antagonistic effect of selenium declined. The antagonistic effect of selenium on the inhibition of neurons＇ survival rate caused by lead wasn＇t observed. Conclusion Lead inhibited the growth and survival of hippocampal neurons. At studied dosage selenium accelerated the growth of hippocampal neu tons and antagonized the lead toxicity at the early culture time.