MCP-1启动子-2518位点基因多态性与云南汉族SLE的研究

Study on the association between MCP-1-2518 promoter polymorphism and systemic lupus erythematosus in Chinese Han in Yunnan Province

目的研究单核细胞趋化蛋白-1（MCP-1）启动子-2518位点基因多态性与云南汉族系统性红斑狼疮（SLE）及狼疮性肾炎（LN）的联系。方法采用聚合酶链反应（PCR）与限制性片段长度多态性（RFLP）方法对61例SLE患者（SLE合并LN患者28例，SLE未合并LN患者33例）和65例正常对照的MCP-1启动子-2518位点基因多态性分布进行分型，以SPSS12．0分析软件分析该基因位点多态性情况。结果各组MCP-1启动子-2518位点基因型A／G较A／A、G／G出现的频率升高；各组MCP-1启动子A／A、G／G及A／G出现的频率无显著性差异（P〉0．05）。结论MCP-1启动子-2518位点基因多态性与SLE、LN的发病和病情之间均无联系。

Objective To investigate the association between systemic lupus erythematosus（SLE） and the polymorphism of MCP-1-2518 promoter in chinese ben patients from Yunnan Province. Methods Genotypes of MCP-1 promoter were determined by polymerase chain reaction （PCR） and restriction fragment length polymorphism （RFLP） in 62 patients with SLE [29 were SLE with lupus nephritis（ LN）, 33 were SLE with no LN ] and 65 normal controls, the difference between each group had been compared. Results The AG genotype was more common in each group. The frequency of A/G was no significant difference among three groups （ P 〉 0. 05 ）. Conclusion These results suggest that there were no association between MCP-1 and the polymorphism in the MCP-1-2518 promoter and SLE or LN.