常温环境中产嗜热蛋白酶菌资源

Resources research on thermophilic protease-producing bacteria in normal temperature environment

通过计数、分离与筛选,对常温环境嗜热菌和产嗜热蛋白酶菌的分布及资源状况进行了研究。结果表明,常温环境中存在着一定数量的嗜热菌和产嗜热蛋白酶菌。土壤与水体相比,其嗜热菌资源相对丰富,且耕作肥沃的土壤中产嗜热蛋白酶菌多于贫瘠土壤;在水环境中,无论湖水、江水还是处理中的废水,在常温条件下均有一定比例的嗜热菌和产嗜热蛋白酶菌。在啤酒废水曝气阶段,产嗜热蛋白酶菌占嗜热菌的比例较大,达45%。本研究筛选的1株嗜热菌其产嗜热蛋白酶活性较高,该菌株在pH7·6、温度68℃条件下其蛋白酶活力达到642U·ml-1。该项研究为开发产嗜热蛋白酶菌资源,在工业和环境治理等方面的应用提供重要科学依据。

The study with routine microbiological methods showed that there existed certain amounts of thermophilic bacteria and thermophilic prrotease-producing bacteria in normal temperature environments. Compared with water body, soil was relatively rich in thermophilic bacterial resources, and thermophilic protease-producing bacteria were richer in fertile than in unfertile soil. In water environment, whether in lake, river and treated waste water, there were definite amounts of thermophilic bacteria and thermophilic proteases-producing bacteria under normal temperature, and the proportion of thermophilic protease-producing bacteria to thermophilic bacteria in the aeration phase of beer wastewater reached 45%, A highly active thermophilic protease-producing strain was screened, with its protease activity reached 642 U ·ml^-1 at pH 7.6 and 68℃. This study was of significance for the exploitation of thermophilic protease-producing bacterial resources, and their application in industry and in environmental protection.