摘要
研究采用L9(34)正交试验,以叶黄素含量(g/kg)为评价指标,用反高效液相色谱法测定,研究了超声温度,超声时间,抗坏血酸用量,超声频率对叶黄素超声提取的最佳工艺条件。试验表明:叶黄素标准曲线的回归方程为Y=1.86×108X-1.74×105,相关系数R2=0.9969,最佳工艺条件为:提取温度40℃,提取时间60min,抗坏血酸用量为7.5%,超声频率为100kHz。该工艺合理、简单、可靠、有效成分提取率高。
According to the orthogonal design L9 （3^4）, four factors in extraction were optimized, i.e. the ultrasonic mance liquid chromatography. The results showed that the regressive equation of the calibration graph was y= 1.86×10^8 x-1.74×10^5, R square value was 0.996 9.The optimum conditions were as follows： extacting temperature is 40 ℃, extracting time is 60 min, the dosage of ascorbic acid is 7.5 % ,supersonic wave frequency is 100 kHz. The process was simple, reliable and high determination. It is reasonable technique.
出处
《食品研究与开发》
CAS
北大核心
2007年第1期97-99,共3页
Food Research and Development
基金
甘肃省自然科学基金(3ZS042-B25-005)
兰州理工大学校基金(SB05200403)
关键词
叶黄素
超声提取工艺
正交试验
反离效液相法
lutein
ultrasound extracting procedure
orthogonal test
reversed-phase high performance liquid chromatography
