大樱桃优质矮化砧木吉塞拉批量组培快繁系统优化的研究

Optimization of Tissue Culture and Rapid Propagation on Sweet Cherry Dwarf Rootstock Gisela in Mass Production

为应用组培技术批量生产优质苗木,以大樱桃优质矮化砧木吉塞拉作为试材,在组培中采用对比试验对如何获得较好的无菌外植体进行了研究.采用正交试验对分化配方和生根配方进行选择,采用多因素对比试验对室外驯化条件进行研究.研究结果表明:外植体材料应在温室自然光条件下培养,以0.1%升汞+0.1%升汞的灭菌方法较为适用;通过正交实验并于生产中验证得到的较佳分化配方为MS+IBA 0.3 mg/L+6-BA 0.8mg/L+白糖30 g/L+琼脂7 g/L,生根配方为MS+IAA 0.3 mg/L+IBA 0.3 mg/L+NAA0.3 mg/L+白糖20 g/L+琼脂6 g/L;采用温室控制光强并保持瓶苗密封处理的生根条件可以提高苗木的驯化成活率.将研究结果应用于生产达到了年批量生产30万棵吉塞拉苗木.

Gisela, which is better dwarf rootstock of sweet cherry, is used as study material. In order to get better quality seedings in mass production by using the technology of plant tissue culture, it has been studied how to get the better bacteria-free explants by contrast tests, to select the better multiplication and rooting culture medium by orthogonal tests, and to get the better domestication conditions in greenhouse on many factors by contrast experiments. The result show that explant material should be cultured at natural condition of greenhouse, the better sterilizing method of getting bacteria-free explants is 0. 1% Hg ＋ 0. 1% Hg, the regenerative culture medium of MS ＋ IBA 0. 3 mg/L ＋ 6-BA 0. 8 mg/L ＋ white sugar 30 g/L ＋ agar 7g/L, the rooting culture medium of MS ＋ IAA 0. 3 mg/L ＋ IBA 0. 3 mg/L ＋ NAA 0.3 mg/L ＋ 20 g/L white sugar ＋ agar 6 g/L are tested the better culture medium＇s formulation by orthogonal tests in both theory and production, and using greenhouse under improve the living Gisela one year by the condition of sealed tissue culture rooting seedlings under controlled light intensity can rate of domestication seedings. There has been match process of 300 thousands using the result in mass production.