贲门癌变过程中Survivin和Cyclin-E蛋白的表达及相关性研究

The expression and correlation of Survivin protein and Cyclin-E protein during cardiac carcinomatous change.

目的检测Survivin蛋白和Cyclin—E蛋白在贲门癌和癌前病变中的表达变化，探讨两者表达的相关性。方法应用免疫组化sP法检测Survivin蛋白和Cyclin—E蛋白在贲门癌和癌前病变组织中的表达变化，分析两者在不同程度贲门病变组织中表达的相关性。结果①从贯门炎到腺上皮不典型增生（Ⅰ、Ⅱ、Ⅲ级）到贲门癌，Survivin蛋白和Cyclin—E蛋白表达均呈逐渐增高趋势，Survivin蛋白的表达率高于Cyclin-E蛋白。②两者在Ⅲ级不典型增生组中的表达与贲门癌组比较差异元显著性（P〉0．05）。③Survivin蛋白和Cyclin—E蛋白在贲门未分化腺癌组中表达均高于其在低分化、高分化腺癌组中表达，组间差异有显著性（P〈0．01）。④Survivin蛋白和Cyclin-E蛋白在贲门炎、腺上皮不典型增生（Ⅰ、Ⅱ、Ⅲ级）和贲门癌中的表达呈正相关（r=0．881，P〈0．05）。结论在贲门不同病变组织中Survivin蛋白和Cyclin-E蛋白表达随着病变严重程度和恶性程度的增高而增高；两者在贲门癌及癌前病变的发生发展过程中起协同作用，共同抑制细胞凋亡、促进细胞增殖。

Objective To detect the expression of survivin protein and cyclin-E protein in cardiac cancer and precancerous lesion t and analyze the relationship between the proteins. Methods SP method was used to detect the expression of survivin protein and cyclin-E protein in cardiac cancer and precancerous lesion. The relationship of their expression in different cardiac lesion tissues was analyzed. Results ①The expression rate of survivin protein and cyclin-E protein from cardiac inflammation to atypical hyperplasia Ⅰ , Ⅱ ,Ⅲ, and then to cardiac cancer increased gradually. In addition, the expression rate of survivin protein was higher than that of cyclin-E protein. ② There was no evident difference in the expression of survivin protein and cyelin-E protein between atypical hyperplasin Ⅲ and cardiac cancer （P 〉 0.05 ）. ③The expression of survivin protein and cyclin-E protein in cardiac non-dif- ferentiation gland cancer was higher than that in low-differentiation and high-differentiation gland cancer （ P 〈 0.01 ）. ④ The expression of survivin protein and cyclin-E protein in cardiac inflammation, atypical hyperplasia Ⅰ , Ⅱ, Ⅲ and cardiac cancer showed positive correlation （ r = 0.881, P 〈 0.05 ）. Conclusion The expression of survivin protein and cyclin-E protein in different cardiac lesion tissues increase gradually with the development of lesion＇s severe degree and malignancy degree. Survivin protein and cyclin-E protein play the same role in the occurrence and development of cardiac cancer and precancerous lesion,both inhabit cell apoptosis and promote cell proliferation.