珍香胶囊抗人宫颈癌作用及其机制研究

Effect and mechanism of Zhenxiang capsule on human cervical carcinoma

目的探讨珍香胶囊对人宫颈癌的抗癌作用及其机制。方法建立人宫颈癌裸鼠移植瘤模型,分别应用TUNEL法和免疫组化SABC法观察不同浓度的珍香胶囊对裸鼠移植瘤凋亡指数(AI)增殖细胞核抗原阳性细胞指数(PI)的影响。结果经珍香胶囊治疗后,高浓度组和中浓度组AI分别为(2．83±1．52)％和(2．50±1．43)％均明显高于阴性对照组的(0．78±0．31)％(P＜0．01,P＜0．05),而低浓度组AI为(0．87±0．27)％与对照组比较,差异无显著性(P＞0．05)；高浓度组和中浓度组PI分别为(63．58±9．31)％和(68．83±9．04)％均低于阴性对照组(84．42±9．25)％(P＜0．01),而低浓度组PI为(79．25±8．53)％与对照组比较,差异无显著性(P＞0．05)。结论珍香胶囊通过抑制肿瘤细胞增殖、诱导肿瘤细胞凋亡而达到其抗人宫颈癌作用。

Objective To investigate the antitumor effect and mechanisms of Zhenxiang capsule（ZXC） on human cervical carcinoma. Methods The impact of ZXC of various concentrations on the apoptosis index （AI）and proliferation cell nuclear antigen （PCNA） - positive nuclei cell iodex（PI） in nude mice with human cervical carcinoma were observed by using the TUNEL detection and SABC method. Results Alter treatment with ZXC the AI in rats of high and intermediate concentration groups was higher than that of negative controls （ P 〈 0.01 or P 〈 0.05）, PI in high and intermediate concentration groups was lower than that of negative group （ P 〈 0.01 ）. Conclusion ZXC possesses antitumor effect on human cervical carcinoma through inhibiting proliferation and inducing apoptosis of tumor cells.