非诺贝特对内皮细胞分泌E-选择素和细胞间黏附分子1的影响

The effects of fenofibrate on endothelial cell secretion of E-selectin and ICAM-1

目的研究非诺贝特对内皮细胞产生E-选择素和细胞间黏附分子1(ICAM-1)的影响。方法培养人内皮细胞株(ECV304),用肿瘤坏死因子α(TNF-α,浓度5、10、25、50、100ng/ml)刺激;用100μM非诺贝特刺激不同时间(1、6、8、12、18、24h)后用10ng/mlTNF-α刺激24h;用不同浓度非诺贝特(0、1、10、100μM)刺激后用10ng/mlTNF-α刺激24h;用ELISA的方法检测培养上清液中的可溶性E-选择素和ICAM-1浓度。结果各浓度的TNF-α刺激24h后E-选择素和ICAM-1浓度明显增加(P<0·05);100μM非诺贝特刺激12、18、24h刺激后抑制E-选择素和ICAM-1分泌增多(P<0·05);用10、100μM非诺贝特刺激后抑制E-选择素和ICAM-1分泌增多(P<0·05)。结论非诺贝特可以抑制E-选择素和ICAM-1分泌。

Objective To investigate the effects of fenofibrate on E-selectin and intercellular adhesion molecule-1（ICAM-1） secretion of the endothelial cells. Methods Cultivated ECV304 cells were stimulated with tumor necrosis factor-α （TNF-α） （5,10,25, 50,100 ng/ml） for 24 hours. The same cells were pretreated with 100μM fenofibrate for different times or cells were pretreated with fenofibrate at different concentration for 24 hours, then these cells were stimulated by TNF-α （10 ng/ml） for 24 hours. Soluble E-selectin and ICAM-1 in the supernatant were measured by ELISA. Results The concentrations of E-selectin and ICAM-1 significantly increased in supernatant of cultivated cells stimulated by TNF-α（P〈0. 05）. The E-selectin and ICAM-1 secreting could be inhibited by fenofibrate. Fenofibrate reduced TNF-ceinduced E-selectin and ICAM-1 expression in a time-and concentration-dependent manner. Conclusion TNF-α fenofibrate can inhibit E-selectin and ICAM-1 secretion of the endothelial cells.