枸杞多糖治疗酒精性脂肪肝大鼠的实验研究

Effect and mechanism of Lycium Barbarum polysaccharide on rat of alcoholic fatty liver

目的观察枸杞多糖(LBP)对大鼠酒精性脂肪肝(AFL)治疗效果并探讨其作用机制。方法采用酒精灌胃建立大鼠慢性AFL模型,选5%和10%的LBP及10%阿托莫兰治疗10W,与酒模组和戒酒组比较:肝脏病理形态,丙氨酸氨基转移酶ALT、天门冬氨酸氨基转移酶AST、γ-谷氨酰基转移酶γ-GT,谷胱甘肽过氧化物酶GSH-PX、超氧化物歧化酶SOD、还原型谷胱甘肽GSH、丙二醛MDA、过氧化氢H2O2。结果LBP能明显改善酒精所致大鼠肝脏脂肪性病理变化,降低肝酶谱,提高GSH-PX和SOD活性,增加GSH含量,减少MDA和H2O2的产生。与阿托莫兰组比较差异无统计学意义,优于单纯戒酒组。结论LBP通过减轻脂质过氧化反应,有效的治疗AFL。

Objective To observe the curative effect and mechanism of Lycium Barbarum polysaccharide(LBP) on the rat of alcoholic fatty liver(AFL).Methods To establish an experimented rat model of chronic AFL through the alcohol intragastric infusion.The rat of AFL were treated with 5% LBP,10% LBP,10% GSH for 10 weeks,then,the pathologic changes were observed.The activity of serum ALT,AST,γ-GT,the activity of liver SOD,GSH-PX and the content of liver MDA,GSH,H_2O_2 were detected.Results LBP could repair the alcoholic-induced fatty pathologic injury,reduce the activity of ALT,AST,γ-GT,and the content of MDA,H_2O_2,increase the activity of SOD,GSH-PX and the content of GSH.The curative effect of LBP was not significantly different compared with GSH,but was more effect than abstinence.Conclusion LBP can treat AFL effectivly by reducing the lipid peroxidation.