高效液相色谱法测定血浆中芬布芬的含量

Determination of Plasma Fenbufen Content by HPLC

目的:建立HPLC法测定芬布芬血浓度。方法:以卡马西平为内标,色谱柱为Hypersil C_(18)柱(200mm×4．6mm,5μm);流速:1．2ml·min^(-1);柱温:45℃;流动相:0．02mol·L^(-1)磷酸二氢钠溶液(含3%三乙胺,用1mol·L^(-1)磷酸溶液调pH 3．00):甲醇=43:57,紫外检测波长284nm。结果:芬布芬的血浓度在0．2～10．0mg·L^(-1)范围内与峰面积比呈良好的线性关系,定量下限为0．2mg·L^(-1)。方法的平均回收率为101．44%±2．3%,内、日间RSD均小于10%。结论:该方法简便、准确,为芬布芬的血药浓度的检测提供了较好的分析方法。

Objective： To establish a HPLC method to determine the plasma content of Fenbufen, Method： The determinate wave-length was 284 nm; The Hypersil C18 colum （200 mm × 4.6 mm,5 μm） was used, The mobile phase was sodium dihydrode phosphoricmethnol（43： 57）. The column temperature was 45℃ and the flow rate was 1.2 mL/min. Result： The determination limit was 0.2 mg·L^-1. The Fenbufen was linear in the range from 0.2 mg·L^-1 to 10.0 mg·L^-1 The RSD was less than 10, 0%. Conclusion： The method is simple,convenient and accurate, which provide the reference methods for determination of blood concentration fo fenbufen.