RP-HPLC法测定热淋清胶囊的槲皮素含量

Determination the Content of Quercetin in Relinqing Capsules by RP-HPLC

目的:建立热淋清胶囊中槲皮素的RP-HPLC测定方法。方法:采用Neuocedur C_(18)柱(250mm×4．6mm,5μm)色谱柱,流动相为甲醇-0．1%磷酸溶液(48:52),流速0．75ml·min^(-1);检测波长为360nm。结果:槲皮素进样量在0．03～0．38μg,与其峰面积呈良好的线性关系(r=0．9999),平均加样回收率为96．5%,RSD为1．1%。结论:该方法简便易行,可用于热淋清胶囊的质量控制。

Objective： To establish an RP-HPLC method to for determinatin the content of quercetin in Relinqing Capsules. Method： The Neuocedur C18 column （250 mm × 4.6 mm,5 μm） was used, the methanol - 0. 1% phosphoric acid solution （48： 52） as mobile phase ; the flow rate was 0.75 ml·min^-1, the detection wavelength was 360 nm. Result： The linear rang of quercetin was 0.03 - 0.38 μg（r =0.999 9）. The average recovery was 96.5% RSD 1.1%. Conclusion： The method is simple, and reliable, it can be used for the quality control of Relinqing Capsules.