血清脂蛋白（a）多克隆试剂ELISA法的方法学初探

PRELIMINARY METHODOLOGICAL STUDY OF POLYCLONE ANTIBODY KIT FOR DETERMINING SERUM Lp(a)

为了将血清Ｌｐ（ａ）测定引入教学并期望在较多临床实验室推广，对南京军区总医院生化科的多克隆试剂盒作了初步的方法学探讨。该法的线性范围在０．０３～０．９６ｍｇ／Ｌ；批内ＣＶ为２．１５％，批间ＣＶ为７．５５％；平均回收率为９７．２％；不受ａｐｏｓＡⅠ、ＡⅡ、Ｂ１００，白蛋白和纤溶酶原等的干扰；与单克隆试剂测定结果比较，相关良好（ｒ＝０．９００７，Ｐ＜０．０１）。对４８份中老年健康者血清Ｌｐ（ａ）测定，其范围在２３～９６０ｍｇ／Ｌ，呈明显的正偏态分布，计算ｘ±Ｓ为１６８±１５５ｍｇ／Ｌ，中位数为１４０ｍｇ／Ｌ；Ｌｐ（ａ）＞３００ｍｇ／Ｌ的检出率为８．３％。

In order to introduce serum Lp(a) measurement into teaching and expecting its application in clinical laboratories domestic polyclone antibody kit for determining serum Lp(a) was studied methodologically. The results showed that the linearity ranges from 0.03～0.96mg/L; the within rum CV and between run CV being 2.15% and 7.55%, and the average recovery rate being 97.2%; it is not influenced by apos AⅠ, AⅡ and B100,albumin and plasminogen. It correlates well with results of serum McAb kit (r=0.9007, P <0.01). Serum Lp(a) of 48 mid and aged healthy subjects determined by the method ranges from 23～960mg/L,showing marked skewed to the left distribution with ±s value of 168±155mg/L and the 50% percentile being 140mg/L, and the detecting rate for Lp(a)>300mg/L was 8.3%.