PCR检测肺结核病人外周血中结核分枝杆菌

DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN THE PERIPHERAL BLOOD OF PATIENTS WITH PULMONARY TUBERCULOSIS USING PCR TECHNIQUE

为了解ＰＣＲ检测外周血中结核分枝杆菌ＤＮＡ对肺结核诊断的价值，采用ＰＣＲ检测了１９０例可疑肺结核病人外周血中结核分枝杆菌ＤＮＡ，并用涂片镜检和培养法检测了这些病人痰中结核分枝杆菌。结果肺结核病人外周血ＰＣＲ阳性率（４４％）明显低于培养法（８０．０％），与涂片法相当（４６．８％）；非肺结核病人外周血ＰＣＲ阳性率为１６．２％（５／３１）。ＰＣＲ检测外周血结核分枝杆菌ＤＮＡ诊断肺结核的应用价值有限。

To evaluate the PCR assay for detecting Mycobacterium tuberculosis in peripheral blood for diagnosing pulmonary tuberculosis.Mycobacterium tuberculosis DNA was detected by PCR technique in the peripheral blood of 190 patients suspected pulmonary tuberculosis,mean while Mycobacterium tuberculosis in the sputa from the patients was examined by microscopic and culture techniques. The results showed that the PCR positive rate(44%,48/109)was obviously less than that of sputum culture (80.0%,87/109),but similar to that of sputum smean method(46.8%,51/109)for patient with pulmonary tuberculosis.Patients without pumonary tuberculosis the PCR positive rate were up to 16.2%(5/31).We believe that detecting Mycobacterium tuberculosis in the peripheral blood of the patients with pulmonary tuberculosis by PCR is of only limited Value for the diagnosis of the disease, It may have some help for the diagnosis of acute active pulmonary tuberculosis and sputum smear negative cases.