PBC患者granulysin基因和蛋白的表达及临床意义

Expression of granulysin in patients with primary biliary cirrhosis and its clinical significance

目的:探讨颗粒溶素(granulysin,GNLY)在原发性胆汁性肝硬化和患者(PBC)中的表达及其意义.方法:采用TaqMan探针技术,以18SrRNA为内参照,测定60例PBC患者外周血中GNLYmRNA的含量,同时应用酶联免疫吸附法(ELISA),检测PBC患者血清中GNLY蛋白的水平,并以健康体检组(n=100)和乙型肝炎肝硬化组(n=60)为对照.结果:PBC组GNLYmRNA的平均拷贝数显著高于健康对照组[(2.7±2.5)×108vs(3.0±1.9)×107,P<0.01]和乙型肝炎后肝硬化组[(2.7±2.5)×108vs(4.7±3.6)×105,P<0.001).PBC患者血清中的GNLY蛋白表达显著高于健康对照组(15.48±3.24μg/Lvs4.76±2.32μg/L,P<0.01)和乙型肝炎后肝硬化组(15.48±3.24μg/Lvs2.57±1.84μg/L,P<0.01).结论:GNLY的表达与PBC的发生、发展存在一定的相关性,可用于PBC临床诊断.

AIM： To explore the serum expression of granulysin mRNA and protein in patients with primary biliary cirrhosis （PBC）. METHODS： Patients with PBC （n = 60）, hepatitis B related cirrhosis （n = 60）, and healthy controls （n = 100） were included in this study. Based on TaqMan fluorescent probe technique, real-time quantitative reverse transcription-polymerase chain reaction （RT-PCR） was performed to detect the expression of granulysin mRNA, using 18S rRNA as the internal control. The level of granulysin protein was measured by immunohistochemistry. RESULTS： The mean copy number of granulysin mRNA was significantly higher in PBC patients than that in healthy controls [（2.7 ± 2.5） × 10^8 vs （3.0 ± 1.9） × 10^7, p 〈 0.01） or patients with hepatitis B related cirrhosis [（2.7 ± 2.5） × 10^8 vs （4.7 ± 3.6） × 10^5, p 〈 0.001）. Meanwhile, the serum level of granulysin protein was also markedly higher in PBC patients than that in healthy controls （15.48 ± 3.24 μg/L vs 4.76 ± 2.32 μg/L） or patients with hepatitis B related cirrhosis （15.48 ± 3.24 μg/L vs 2.57 ± 1.84 μg/L, P 〈 0.01）. CONCLUSION： Granulysin expression is associated with the occurrence and progression of PBC, which can help to make diagnosis for PBC.