胃癌组织中MK、VEGF表达的意义

Expression of midkine and vascular endothelial growth factor and their significances in gastric carcinoma

目的:研究中期因子(midkine,MK)和血管内皮生长因子(VEGF)在胃癌组织中的表达情况,并探讨其临床意义.方法:采用免疫组织化学方法检测64例胃癌组织、20例癌旁正常组织中MK、VEGF和CD34蛋白表达情况.结果:胃癌组织中MK、VEGF的阳性表达率分别为76.6%和79.7%,显著高于癌旁正常组织(0,15.0%)(P<0.05).MK、VEGF的表达水平与胃癌的浸润深度(χ2=7.111,P=0.008;χ2=7.590,P=0.006)、淋巴结转移(χ2=4.814,P=0.028;χ2=6.207,P=0.013)、临床分期(χ2=13.971,P=0.001;χ2=18.554,P=0.000).MK表达阳性的胃癌组织MVD显著高于MK表达阴性的胃癌组织(31.745±8.592vs24.680±8.938,P<0.01),而且MK、VEGF表达在胃癌中也存在一定相关性(χ2=4.447,P<0.05).结论:MK、VEGF在胃癌的生长、侵袭与转移中起重要作用,他们之间存在相互诱导或是协同效应,其共同表达可作为判断胃癌预后的指标.

AIM： To investigate the expression vascular endothelial growth factor （VEGF） and midkine （MK） and their significances in gastric carcinoma. METHODS： Immunohistochemistry was performed to detect the expression of MK, VEGF and CD34 in gastric carcinoma （n = 64） and adjacent normal tissues （n = 20）. RESULTS： The positive rates of MK and VEGF expression were significantly higher in gastric cancer tissues than those in the normal ones （76.6%, 79.7% vs 0, 15.0%, both P 〈 0.01）. The expression of MK and VEGF had marked correlations with the invasion depth （X^2 = 7.111, P = 0.008; X^2 = 7.590, P = 0.006）, lymph node metastasis （X^2 = 4.814, P = 0.028; X^2 = 6.207, P = 0.013）, and clinical stages （X^2 = 13.971, P = 0.001; X^2 = 18.554, P = 0.000）. The value of MVD in patients with positive MK expression was notably higher than that in ones with negative MK expression （31.745 ± 8.592 vs 24.680 ± 8.938, P 〈 0.01）, and there was a positive correlation between MK and VEGF expression （X^2 = 4.447, P 〈 0.05）. CONCLUSION： MK and VEGF may play synergistic roles in the growth, invasion and metastasis of gastric carcinoma, and their co-expression may be a useful indicator for the prognosis of gastric carcinoma.