摘要
利用AFLP技术对我国国家草莓资源圃内保存的107个草莓品种进行了DNA多态性分析。从64对“2+2”引物中筛选出10对引物应用于扩增基因组DNA,共获得清晰可辨的581条带,其中412条为多态性带。UPGMA方法聚类分析结果显示,AFLP分子鉴定结果与其系谱关系非常一致,表明AFLP指纹图谱分析能很好地鉴别草莓品种之间的遗传关系。
The AFLP analysis was conducted in order to investigate the blood relationship among the 107 strawberry cultivars conserved in Beijing, the National Strawberry Germplasm Repository. 10 pairs primers were selected from 64 primer pairs （2 ＋ 2 ） to amplify the genomic DNAs. Total 581 AFLP fragments were obtained, 412 of them were polymorphic markers. The phylogenetic tree generated by UPGMA method showed the genetic relationship among cultivars identified by AFLP was better identical with their pedigree. AFLP technique was proved to be a very reliable method to study the genetic relationships of strawberry cultivars.
出处
《园艺学报》
CAS
CSCD
北大核心
2006年第6期1199-1202,共4页
Acta Horticulturae Sinica
基金
北京市科委资助项目(H012010390113)
关键词
草莓
AFLP
亲缘关系
分子鉴定
Strawberry
AFLP
Genetic relationship
Molecular identification
