花烛体细胞胚胎发生及植株再生研究

Somatic Embryogenesis and Plant Regeneration of Anthurium andraeanum

以花烛(Anthurium andraeanumLind.)盆花品种无菌苗叶片、叶柄、根段为外植体,对花烛体细胞胚胎发生及植株再生进行了研究,建立了花烛体细胞胚胎发生体系。结果表明,花烛叶片较其他外植体更适宜体细胞胚的诱导;胚性愈伤组织及胚性结构诱导最适培养基为改良MS+2,4-D2.0～3.0mg.L-1+KT0.5mg.L-1+蔗糖2%+葡萄糖1%,pH5.5,暗培养40d,诱导率可达90.3%;体胚发育适宜培养基为改良MS+6-BA0.5mg.L-1+蔗糖3%,光强20μmol.m-2.s-1,培养20d后体胚萌发率达62.9%;萌发的体胚在发育培养基上继续培养30d后均能发育成完整植株。对不同阶段培养材料的组织结构及超微结构的观察证明了花烛体胚发生过程。

A protocol for somatic embryogenesis and plant regeneration of Anthurium andraeanum Lind. in vitro plants has been successfully developed. Leaf sections, petioles and root sections were used as explants, the results showed that leaf sections were suitable explants and expressed higher embryogenic potential than others; Up to 90. 3% of explants produced embryogenic caUi and embryogenic structures after 40 days culture on modified MS medium supplemented with 2. 0 to 3.0 mg·L^-1 2,4-D, 0. 5 mg · L^-1 KT, 2% sucrose and 1% glucose; 62. 9% of somatic embryos developed after 20 days culture on modified MS medium containing 0. 5 mg·L^-1 6-BA and 3% sucrose under light conditions of 20μmol· m^-2 ·s^-1 and converted into plantlets with shoots and roots after 30 days culture on the same medium. The observation of histological structure and ultrastructure proved the process of somatic embryogenesis of Anthurium.