2种核酸染色方法的比较

A Comparative Study on Two Staining Methods of Nucleic Acid

目的:比较前染和后染等2种核酸染色方法对凝胶中DNA的染色效果,及其对染料染色灵敏度的影响,并验证新型核酸染色剂SYBRGreenⅠ能否代替传统染料溴化乙锭(EB)用于常规电泳中DNA的染色。方法:分别用SYBRGreenⅠ与EB采用2种不同的方法对凝胶中的DNA进行染色。结果:前染和后染的染色效果无显著差别,2种染料都能显示出10ng以下的DNA条带。结论:前染和后染方法的染色效果相当,染料SYBRGreenⅠ和EB均可用于这2种染色方法;新型染料SYBRGreenⅠ可代替强致癌性染料EB,用于常规凝胶中DNA的染色。

Objective： To compare the effect and their sensitivity of two different staining methods to DNA in the gels, and evaluate the possible application of SYBR Green Ⅰ in routine DNA staining as a substitute for ethidium bromide （EB）. Methods： The new nucleic acid staining SYBR Green Ⅰ and EB were used to stain the DNA in the gel, perfectively stain before electrophoresis and after electrophoresis. Results： There was no distinct difference between the two methods. Both SYBR Green Ⅰ and EB stain could detect as little as 10 ng per band of DNA. Conclusion： The effect and sensitivity of the two staining methods are no distinct difference, and SYBR Green Ⅰ and EB may be used in these staining methods. SYBR Green Ⅰ, which is a new nucleic acid stain may substitute for EB as a general dyestuff to stain the DNA in the gel.