严重创伤对小鼠体内单核细胞向树突状细胞分化及迁移过程的影响

Effect of severe trauma on differentiation and migration of monocytes to dendritic cells

目的观察严重创伤对小鼠体内单核细胞向树突状细胞分化及迁移的影响。方法皮下注射FITC荧光微球,细胞计数,流式细胞仪检测等观察引流淋巴结荧光阳性细胞数量及所占比例;荧光显微镜观察注射局部炎性细胞浸润情况及局部组织荧光强度;丙酮提取法检测局部残余荧光物质并计算局部荧光物质清除率。结果严重创伤动物引流淋巴结FITC荧光阳性细胞百分比(0．021±0．009)％及绝对数量[(492±211)个]均显著低于正常对照组动物[(0．040±0．012)％,P<0．05;(726±218)个,P<0．05];伤后早期创伤动物微球注射局部炎性细胞浸润明显增强;严重创伤动物耳片荧光微球清除率在注射微球后1、2、4和6天均显著高于正常对照组动物,差异显著(P<0．05)或非常显著(P<0．01)。结论严重创伤后小鼠体内单核细胞向树突状细胞的分化并迁移的过程发生了障碍,该障碍并非是由于单核细胞浸润炎性病灶和否噬细胞摄取抗原及迁移启动受阻所致。

Objective To observe the effect of serious trauma of mice on differentiation and migration of monocytes to dendritic cells. Methods FITC-labeled polystyrene microspheres were injected subcutaneously, the total ceils of draining lymph nodes were counted and analyzed by flow cytometry. Local infiltration of inflamed ceils and remaining fluorescent substances were observed by fluorescence microscopy. Remaining fluorescent substances were measured by acetone extractions test, and then the clearance rates were calculated. Results The percentage and quantity of FITC^＋ cells in draining lymph nodes of sever trauma animals [ （ 0. 021 ±0. 009 ） % , （ 492 ±211 ） cells ] was significantly lower than that of control animals [ （ 0.040 ±0.012 ） %, （ 726 ±218 ） cells ] （ P 〈 0.05 ）. During early period after trauma, the infiltrations of inflamed cell at injection area were seen more obviously. The fluorescence clearance rates of severe trauma animals were significantly higher than that of control animals （ P 〈 0.01 or P 〈 0.05 ） on dayl ,2,4 and 6 post microspheres injection. Conclusion The disorder of differentiation and migration of monocytes to dendritic cells in vivo occurred in mice after trauma, which is not attributed to the depression of both monocytes infiltration to inflamed area or antigen uptake and migration starting of phagocytic cells.