大鼠骨髓间充质干细胞定向诱导为血管内皮样细胞

Bone marrow mesenchymal stem cells induced into endothelium like cells

①目的探讨体外分离和纯化大鼠骨髓间充质干细胞体外定向诱导分化为血管内皮样细胞及其为组织工程血管的构建提供种子细胞的可能性。②方法收集大鼠股骨骨髓血。进行体外扩增培养成纯化的间充质干细胞，取第4—6代生长状态良好的间充质干细胞，用含40ng／mL内皮细胞生长因子（vascular endothelial prowth factor，VEGF）培养液诱导细胞，利用免疫组织化学观察vWF的表这。③结果通过离体培养可使体内环境下低农度的骨髓间充质干细胞实现数目扩增；诱导14天后实验组细胞vWF强阳性表达，接近汇合的细胞外观呈现特征性的鹅卵石样形态。对照组细胞vWF呈阴性表达。④结论体外可以获得足够量的较为单一、具有较强增殖能力的间充质干细胞，而间充质干细胞可以在体外定向分化为具有内皮血管细胞特性的细胞。

Objective To explore the possibility of bone marrow mesenchymal stem cells （BMMSCs） isolated and induced into endothelium like cells in vitro, and provide stem cells for the construct of blood vessel in tissue engineering. Methods The marrow of thighbone in rats were collected and cultured into the rarefied BMMSCs. BMMSCs of 4 - 6 passages were induced by 40ng/ml vascular endothelial growth factor （ VEGF）, then the characteristics of cells vWF was analysed by immunohistochemistry. Results The number of BMMSCs was proliferated by incubation in vitro. After 14 days, expression of vWF was positive in the experiment group, the confluent cells showing a morphology like cobblestone, while which was negative in the control group. Conclusion The purified BMMSCs with ability of multiplication could be obtained and induced directionally into endothelium like cells in vitro.