实时荧光定量RT-PCR分析直肠癌PPARδ的表达及意义

Quantification of PPARδ mRNA by Real-time RT-PCR in Rectal Cancer Tissues

目的分析人直肠癌组织过氧化物酶体增殖物活化受体δ基因（PPAR8）的表达变化及其与直肠癌临床病理特征的关系。方法选取手术切除的直肠癌标本86例，以癌旁正常粘膜为对照，采用实时荧光定量RT-PCR对PPARδmRNA作定量分析。结果48例（55．8％）直肠癌组织PPAR8表达上调，其中39例（81．3％）上调1．5～5．0倍，5例（10．4％）上调10-20倍，4例（8．3％）大于20倍，与对照组相比，两组间差异无统计学意义（P=0．083）。PPARδ的表达与直肠癌分化程度、病理类型及Dukes分期无相关性（P〉0．05）。结论直肠癌组织中PPAR8基因的表达无显著上调，并且与直肠癌分化程度、病理类型及Dukes分期无关。

Objective To clarify the expression change of PPARδ gene in human rectal cancer tissues and determine the correlation of PPARδ expression with the clinical and pathological parameters of rectal cancer. Methods Applying real-time RT-PCR, we quantified PPARδ mRNA in 86 tissues from excised primary rectal cancers. In each ease, accompanying normal mucosa was collected for comparison. Results Among the 86 rectal cancer tissues, 48 （55.8%） eases showed PPARδ overexpression： 39 （81.3%） tumors gave an expression level 1.5 to 5.0 times, 5 （10. 4%） tumors 10 to 20 times, and 4 （8.3%） tumors more than 20 times relative to normal mucosa. However, the general level of PPARδ mRNA in rectal cancer tissues is not statistically different from that in normal mucosa. There was no evidence for the relationships of PPARδ expression with cell differentiation, pathological categories and Dukes stages. Conclusion The expression of PPARδ gene in rectal cancers is not statistically different from that in normal mucosa, and it is not correlated with cell differentiation, pathological categories and Dukes stages.