鹿茸精诱导大鼠骨髓间充质干细胞分化为神经元样细胞

Rat mesenchymal stem cells differentiate into neuron-like cells induced by pantocrine in vitro

目的:探讨鹿茸精体外定向诱导SD大鼠骨髓间充质干细胞分化为神经元样细胞。方法:通过贴壁法分离大鼠骨髓间充质干细胞,体外扩增培养。鹿茸精定向诱导分化为类神经元样细胞。光镜下观察细胞形态,免疫细胞化学检测神经细胞特异性抗原标志物神经元烯醇酶(NSE)、巢蛋白(Nestin)、胶质纤维酸性蛋白(GFAP)的表达。结果:大鼠骨髓间充质干细胞可通过贴壁法成功分离并可在体外大量扩增。鹿茸精诱导3小时后大部分骨髓间充质干细胞转变为神经元样细胞,出现胞体和突起,免疫细胞化学染色NSE及Nestin呈阳性,GFAP阴性。结论:鹿茸精可在体外诱导大鼠骨髓间充质干细胞分化为神经元样细胞。

Objective：To investigate whether pantocrine can induce rat mesenchymal stem cells （MSCs） to differentiate into neuronlike cells in vitro.Methods：Mesenchymal stem cells were separated from thigh bone marrow of rats by wall sticking method, amplifying cultured in vitro,and differentiated into neuronlike cells by oriented induction with pantocrine.The morphology of cells was observed under light microscopy,neuron-specific enolase （NSE）,nestin and glial fibrillary acidic protein （GFAP） were detected by imnunocytoehemistry. Results：Rat mesenchymal stem cells could be separated and amplified in vitro.After being induced by pantocrine for 3 hours,mest of the cells would be differentiated into meuron-like cens,reveuling cytodendrite.By immunochemical staining, cells showed positive of NSE, nestin, and negative of GFAP.Conclusion：Rats mesenchymal stem cells could be induced to differentiate into neuron-like cells by pantocrine.