nm23－H_1基因在人乳腺癌组织中的表达及其与淋巴结转移的关系

Expression of nm23-H1 gene and its correlation with lymph node metastasis in human breast cancer

目的探讨一２３－Ｈ；基因在人乳腺癌和良性乳腺疾患中的表达规律．及其与淋巴结转移等预后因素之间的关系．方法乳腺组织标本共７６例，ＨＥ染色对病变进行病理诊断，以且抗人ｕｒｎ刀一写单克隆抗体ＩｇＧ。、用免疫组化方法检测ｎ。２３－Ｈ；基因在乳腺组织中的表达产物．结果①＂ｍ２３－Ｈ；表达产物位于胞浆；②＂ｍ２３一马表达频率在良性乳腺疾患中明显高于乳腺癌中；③＂ｍ２３一ｑ的表达与乳疗淋巴结转移负相关．结论＂ｍ２３－Ｈ；的检测在评估乳腺肿瘤的恶性程度及淋巴转移上有肯定的意义，且是评估乳腺癌患者预后有价值的生物学指标．

Objective To research expression of thenln23-- H, gene in human breast ca￣ andbenign breast diseases, and its correlation withlymph node metastasis and other prognostic factors.Methods After Histologic typing was determined by the study of sliders that had been stainedwith hematoxylin and eosin in 76 PahentS withbreast disease. By using halnunohistochehealmethods and mouse monoclonal IgG. antibodyto 'm23--H, gene, produCtion of 'm23--H, expression of 76 breast tissue sampleS was deteCted.Results (1) tun23--H, gene product was inthe cytoplasm. (2) The freguence of'm23--H, gene expressinn was higher in breastbenign lessions than that in breast cancers. (3)'m23-- H, expression was negative correlatinnwith lymph nodal metustaSis of breast cancer.Conclusion Detection of nln23--HI expreSsionmay have certain inPOrtance in the evaluahon ofgreat malignante degree and lymphatic metaStasisof breast neoplasmas. alxl inn23--H, gene is avaluable biologic marker for det￣ng Prognosisof PatientS with breast cancer.