大肠癌组织C-Ki-ras基因突变的研究

THE STUDIES OF C-Ki-ras MUTATIONS IN COLORECTAL CANCER

应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)研究了大肠癌组织中C-Ki-ras基因的突变情况.结果显示:大肠癌组织中C-Ki-ras基因第12位密码子的突变频率为53.6%;而C-Ki-ras基因第13位密码子的突变频率为18%.结果提示C-Ki-ras基因的突变可以做为大肠癌诊断的一个极好的分子遗传学指标.

A sensitive restriction fragment length polymorphism assay was used to detect C - Ki-ras mutations in 110 specimens of colorectal epithelium from 110 patients with colorectal cancer. The results show that the mutation frequency of the C - Ki - ras codon 12 is 53. 6% ,whereas the mutation frequency of the C -Ki - ras codon 13 is 18%. These studies in- dicate that mutations of C -Ki -ras may be an excellent molecular genetic marker to diagnosis colorectal cancer.