白杨叶绿体和线粒体DNA的多态性及遗传性分析

Genetic characters and polymorphism of chloroplast and mitochondrial DNA in white poplars.

利用PCR-RFLP技术对白杨派毛白杨、毛新杨和银腺杨及其F1代的叶绿体和线粒体遗传进行了分析.以总DNA为模板,扩增了叶绿体trnD-trnT、rps12/7-nadB、trnT-trnF、trnL-trnF3′和trnL-trnF5′基因片段,线粒体coxⅢ、ND5/6、orf25、atp6、nad4/1-2和ND2-COⅢ基因片段,比较了这些扩增片段5种限制性内切酶的酶切片段多态性.结果表明毛新杨×毛白杨、毛新杨×银腺杨F1代的叶绿体DNA为母性遗传;4种杂交组合线粒体基因片段的PCR扩增与酶切图谱完全一致,未发现多态性,表明白杨线粒体基因高度保守.

The inheritance of chloroplast and mitochondrial DNA of Populus tomentosa, P. tomentosa x P. bolleana and P. alba × P. glandulosa as well as their FI progenies were analyzed by PCR-RFLP. The chloroplast trnD-trnT, rps12/7-nadB, trnT-trnF, trnL-trnF3＇ and trnL-trnF5＇, together with six mitochondrial genes, cox Ⅲ, NDS/6, orf25, atp6, nad4/1-2 and ND2-CO Ⅲ, were amplified from these strains respectively ; cpDNA and mtDNA polymorphism of these amplified fragments were compared among parental strains by restriction digestion. Restriction fragment length polymorphism （RFLP） analysis revealed that all the chloroplast DNA in FI individuals of （ P. torrtentosa × P. bolleana ） × P. tomentosa, （ P. tomentosa × P . bolleana ） × （P. alba × P . glandulosa ） were the maternal inheritance. Moreover, there are no variations among these four hybrids in the PCR amplification pattern and the restriction pattern, indicating that mitochondrial genes are highly conserved in white poplars.