NaOH处理结缕草种子对种带真菌的影响

Effect of Sodium Hydroxide Treatment on Seed-borne Fungi of Zoysia japonica

用20%NaOH处理2002、2003和2004年收获的结缕草(Zoysiajaponica)种子,采用PDA培养法测定种子带菌率、种带真菌的种类和分离频率。结果表明:NaOH处理结缕草种子降低其带菌率,与表面消毒效果相当,处理后随着贮藏时间的延长,种子带菌率升高。经过NaOH处理的种子再进行表面消毒效果不明显。在供试材料中共检出25属34种真菌,其中曲霉、青霉的分离频率最高。用NaOH浸泡和表面消毒种子均可以降低其带菌种类,且二者效果大致相同。

We studied the seed-borne fungi of Zoysia japonica for safe seed treatment and storage. Zoysia japonica seeds harvested in 2002, 2003, and 2004 were treated with 20% sodium hydroxide solution. To detect seed-borne fungi on/in the seed samples, PDA plate method was used. The results show that soaked in 20% sodium hydroxide solution, the seed reduced in the percentage of infection of seed-borne fungi; the effect was similar to seed surface sterilization. However, with prolonged storage time, the percentage of seed-borne fungi rose. Surface sterilization of previously sodium hydroxide treated Zoysia japonica seeds proved to be of no marked effect. On/in the samples, 34 fungi species of 25 genera had been identified. Among them, Penicillium spp. and Aspergillus spp. were the more frequently distinguished. Seed soaked in 20% sodium hydroxide solution or undergone surface sterilization attain to similar effect of reducing percentage of fungi infection and of fungi species. The two methods achieve similar results.