流动注射-抑制化学发光法测定雷尼替丁

Determination of ranitidine by flow injection suppressive chemiluminescence

目的:建立雷尼替丁含量测定的流动注射化学发光新方法。方法:基于在弱碱性的多聚磷酸钠介质中,雷尼替丁对二价铜离子-过氧化氢-罗丹明B化学发光体系具有强烈的抑制作用而建立的一种新的流动注射化学发光分析法。结果:发光信号的降低值(ΔI)与雷尼替丁的质量浓度在5．0～1000μg·L^(-1)范围内呈良好的线性关系,平均回收率为98．9%～102．9%。对100μg·L^(-1)的雷尼替丁连续进行11次平行测定,其相对标准偏差为3．0%。根据IUPAC建议,计算其检出限为2．7μg·L^(-1)(3σ)。结论:该法可用于胶囊中雷尼替丁含量的测定。

Objective： To establish a novel flow injection chemiluminescence （FI-CL） system for determination of ranitidine. Methods： Ranitidine was used as a suppressor to decrease chemiluminescent intensity in a reaction of Cu^2＋ with H2O2 and radicals oxidized rhodamine B in sodium polyphosphate media. Results： The linearity of the ranitidine concentration curve versus reduction value of the CL intensity （ △I） was found when ranitidine concentration was within 5.0 - 1 000μg·L^-1. The determination concluded a mean recovery of ranitidine of 98.9% - 102.9% , a detection limit of 2.7μg·L^-1 （3σ） and the relative standard deviation （RSD） of 3.0% （n =11, ranitidine of 100μg·L^-1）. Conclusion： This sensitive and reliable FL-CL method can be used in quantification of ranitidine capsules.