高效液相色谱荧光法测定人血浆中盐酸伐昔洛韦的代谢产物阿昔洛韦

Quantification of acyclovir in human plasma——the metabolite of valaciclovir hydrochloride by high performance liquid chromatography

目的:建立高效液相色谱荧光分析方法测定人血浆中盐酸伐昔洛韦的代谢产物阿昔洛韦(ACV)。方法:20例受试者单次、交叉口服盐酸伐昔洛韦片300mg后,以喷昔洛韦为内标,用沉淀法去除蛋白,用HPLC-荧光法测定。结果:ACV在0．02～5μg·mL^(-1)的浓度范围内有良好的线性关系(r=0．999 95),最低检测浓度为0．02μg·mL^(-1)(S/N＞3),ACV的相对回收率为96．08%～97．28%,绝对回收率为69．62%～72．02%(n=5),日内精密度RSD为3．33%～6．12%,日间精密度为2．37%～6．81%。结论:本方法简便、灵敏、特异性强,可用于血浆中ACV的测定及人体药动学研究。

Objective： To establish a HPLC-FLD method for determination of plasma concentration of acyclovir that is a metabolite of valaciclovir hydrochloride. Method： 20 healthy volunteers in a randomized crossover study was orally taken with a single oral dose of valacyclovir hydrochloride tablet 300 mg. The human plasma samples were collected and purified by precipitation to measure the acyclovir concentration by HPLC. Pencilcovir was used as an internal standard. Results： A calibrated linear curve of acyclovir concentration was within 0.02 - 5 μg·mL^-1 （ r = 0. 999 95 ）. The low detection limit of acyclovir was 0.02μg·mL^-1 （ S/N 〉 3 ）. The relative and absolute recovery rate of acyclovir was 96. 08% - 97.28% and 69. 62% - 72. 02% （n = 5）, respectively. The within-day RSD and among-day RSD of acyclovir were 3.33% - 6. 12% versus 2. 37% - 6. 81%. Conclusion： This reliable and sensitive HPLC method was applied to monitor plasma aciclovir concentration as well as pharmacokinetic outcomes.