摘要
目的探讨芳香化酶P450(P450arom)在子宫内膜增生症、子宫腺肌病等雌激素依赖性疾病病变内膜中的不同表达。方法采用免疫组化方法分别测定子宫腺肌病异位内膜(Ⅰ组)、子宫腺肌病在位内膜(Ⅱ组)、子宫内膜增生症内膜(Ⅲ组)、正常子宫内膜(Ⅳ组)四组不同内膜中P450arom的表达。根据阳性率和表达强度进行量化评分(免疫组化评分)。结果①P450arom在子宫腺肌病异位、在位、子宫内膜增生症内膜中均阳性表达,但在正常子宫内膜无表达,P450arom在异位子宫内膜表达强度明显高于在位子宫内膜、增生症子宫内膜(P<0.05);异位子宫内膜病灶周围肌肉组织中也有P450arom表达;②P450arom在子宫内膜异位症内膜的增殖期和分泌期表达,差异无统计学意义(P>0.05)。结论①雌激素限速酶P450arom是子宫腺肌病、子宫内膜增生症等雌激素依赖性疾病发生、发展的重要激素,局部分子水平代谢紊乱可能导致疾病发生。②内膜P450arom检查不能作为盆腔子宫内膜异位症诊断方法,但可作为雌激素依赖性疾病筛选方法。
Objective To investigate the expression of P450 aromatase in estrogen - dependent gynecologic diseases, such as endometrial hyperplasia and adonomyosis. Methods P450arom was detected immunohistochemically in the four experimental groups： endometrial hyperplasia, adonomyosis and eutopic endometrium of women with or without endometriosis. Results ① P450aroma was expressed in eutopic and ectopic endometrium of adonomyosis, endometrial hyperplasia, but negative in endometrium without pathological changes, The expression of P450 aromatase in ectopic endometrium was stronger than that of eutopic endometrium （P〈0. 05）. P450 aromatase was expressed in the smooth muscle tissues around ectopic endometrium also. ② No difference of P450 aromatase expression was found between proliferative and secretory endometriurn of adonomyosis （P〉0.05）. Conclusion ① Aromatase p450 is the key enzyme for biosynthesis of estrogen, which is an essential hormone for the orientation and development of the estrogen - dependent gynecologic diseases. The pathological changes is due to molecular aberrations on the local metabolism;② Although P450 aromatase is not a diagnostic test for pelvic endometriosis, but it may be regarded as a method of screening the estrogen-dependent gynecologic diseases.
出处
《中国妇产科临床杂志》
2007年第1期23-25,64,I0001,共5页
Chinese Journal of Clinical Obstetrics and Gynecology
