复方黄黛片含药灭活兔血清对NB_4细胞株的作用

Effects of inactivated rabbit serum containing compound realgar and natural indigo tablet on cell line NB_4

目的:研究复方黄黛片含药灭活兔血清对人急性早幼粒细胞白血病NB4细胞株的作用。方法:以兔为实验血清的供体,将兔血清灭活,以灭活前给药与否为分组依据,采用双道原子荧光法检测血清中砷的浓度,以细胞抑制率及凋亡率为观察指标,对实验结果进行分析。结果:给药前、后灭活兔血清中砷浓度分别为(0.0100±0.0010)mg/L和(0.1100±0.0064)mg/L,差异有统计学意义(P<0.01);两组兔血清对NB4细胞株生长均有抑制作用,且对NB4细胞株生长的抑制作用具有一定的浓度及时间依赖性,实验组间,各浓度及时间组间差异有统计学意义(P<0.01)。两组兔血清均可诱导NB4细胞株发生凋亡,且该作用具有一定的浓度及时间依赖性,实验组间,各浓度及时间组间差异有统计学意义(P<0.01)。结论:复方黄黛片含药灭活兔血清可明显抑制NB4细胞株的生长,抑制作用呈一定的浓度、时间依赖性。复方黄黛片含药灭活兔血清也可诱导NB4细胞株发生凋亡,作用也呈一定的浓度、时间依赖性。

Objective. To explore the effects of inactivated rabbit serum containing compound realgar and natural indigo tablet （CRNIT） on cell line NB4. Methods. The experimental rabbits were taken as the provider of the animal serum, and the serum was inactivated before the experiment. The serum was divided into two groups based on whether the rabbits were given CRNIT. The concentration of arsenic in the rabbit＇s serum was detected by AFS-230a double path atom fluorescence photometer. The inhibition rates and apoptosis rates were regarded as the observational indexes. Results. The concentration of arsenic in the inactivated rabbit serum containing and not containing the drug were （0. 010 0 ±0. 001 0） mg/L and （0. 110 0±0. 006 4） mg/L respectively, and the difference had statistical significance （P〈0.01）. The two groups of serum all had inhibitory effect on the growth of NB4 cells depending on the drug concentration and effect time. And there were significant differences among the groups. The two groups of serum all induced the apoptosis of NB4 with positive relations with the concentration and effect time. And there were significant differences among the groups. Conclusions： The rabbit serum containing CRNIT can obviously restrain the growth of NB4 cells and the inhibitory effect depends on the concentration and effect time. And the rabbit serum containing CRNIT can also induce the apoptosis of NB4 cell line and the apoptosis rates depend on the concentration and effect time.