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高通量、多组分表面等离子体谐振生化分析系统研究 被引量：2

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摘要 基于表面等离子体谐振(surface plasmon resonance,SPR)原理,利用图像分析技术和自动进样技术,研制成一种新型高通量、多组分图像SPR生化分析系统;对微阵列SPR敏感芯片进行动态检测,实现高通量、多参数、多组分快速检测和定量分析．以15单元的阵列芯片为例,对兔IgG和人IgG分别与羊抗兔IgG和羊抗人IgG的免疫结合反应进行了实验检测．结果表明,该分析系统可进行实时高通量、多参数检测,具有灵敏度高、免标记等优点,而且阵列芯片中所设置的参比单元可以消除溶液本体折射率和温度变化的影响,提高了测量精度和准确性;另外芯片可以再生重复使用,降低了测试成本．
作者 崔大付 梁金庆 蔡浩原 王军波 李亚亭 王于杰 刘长春 郑自攀
机构地区 中国科学院电子学研究所传感技术国家重点实验室
出处 《自然科学进展》 北大核心 2007年第1期93-98,共6页
基金 国家自然科学基金重点项目(批准号:60427001)中国科学院仪器研制项目(批准号:Y2004005)资助
关键词 SPR高通量 多通道 微阵列
分类号 O657.3 [理学—分析化学]
  • 相关文献

参考文献11

  • 1Homola J.Present and future of surface plasmon resonance bio sensors. Analytical and Bioanalytical Chemistry, 2003, 377(3):528 539.
  • 2崔大付,李向明,蔡浩原,李亚亭,郑自攀,陈翔.表面等离子体谐振(SPR)生化分析仪的研制[J].现代科学仪器,2001,18(6):34-38. 被引量：32
  • 3崔大付，周力夫．表面等离子体谐振装置．国家发明专利，ZL98102366．5，2002年8月.
  • 4崔大付,王于杰,蔡浩原,向四海,张国青,王晋芳,钱世钧.SPR生化分析仪对6-氧甲基鸟嘌呤-DNA甲基转移酶的检测[J].自然科学进展,2003,13(8):874-876. 被引量：16
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二级参考文献16

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共引文献41

同被引文献17

  • 1梁金庆,崔大付,蔡浩原,王军波,王于杰.利用图像SPR分析仪检测多组分蛋白芯片[J].传感器与微系统,2006,25(10):57-59. 被引量：3
  • 2陈悦,崔大付,蔡浩原,王军波,王于杰.小型SPR生物传感器的研制[J].传感器与微系统,2006,25(12):79-81. 被引量：3
  • 3Hayes T B, Collins A, Lee M, et al. Hermaphroditic, demasculinized frogs after exposure to the herbicide atrazine at low ecologically relevant doses[ J]. Proc Natl Acad Sci U S A, 2002,99(8):5476-5480.
  • 4Indyk Harvey E, Filonzi Enrteo L. Determination of lactoferfin in bovine milk, eolostrum and infant formulas by optical biosensor analysis [ J ]. International Dairy Journal ,2005,15 ( 5 ) : 429 - 438.
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  • 6Wu C M, Lin L Y. Immobilization of metallothionein as a sensitive biosensor chip for the detection of metal ions by surface plasmon resonance [ J ]. Biosens Bioelectron ,2004,20 (4) : 864- 871.
  • 7Ateeq Ahmad, Anand Ramakrishnan, Matthew A McLean, et al. Use of optical biosensor technology to study immunological cross-reactivity between different sulfonamide drugs[J]. Analytical Biochemistry ,2002,300(2): 177-184.
  • 8Akkoyun, Kohen V F, Bilitewski U. Detection of sulphamethazine with an optical biosensor and anti-idiotypic antibodies[ J]. Sensors and Actuators B,2000,70 ( 1 - 3 ) : 12-18.
  • 9Bjurling P, Baxter G A, Caselunghe M, et al. Biosensor assay of sulfadiazine and sulfamethazine residues in pork [ J ]. Analyst, 2000,125 (10) : 1771-1774.
  • 10崔大付,周立夫.表面等离子体谐振装置[P].国家发明专利,ZL98102366.5.

引证文献2

二级引证文献13

自然科学进展
2007年 第1期

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