瘢痕疙瘩家系外周血B淋巴母细胞系的建立方法及意义

Establishment of B-lymphoblastoid cell lines from peripheral blood of keloid pedigree and its significance

目的:探讨建立瘢痕疙瘩家系永生化细胞库的方法,为研究瘢痕疙瘩的发病机制、诊断和治疗提供长期的标本来源.方法:采用CysA法、微量全血法和冻存全血法3种不同方法建立B淋巴母细胞样细胞系(B-LCL),比较各方法建系成功率及时间.结果:成功建立了一瘢痕疙瘩家系的永生细胞株,其中CysA法、微量全血法和冻存全血法转化的成功率分别为96.4%,46.4%,28.6%;建立细胞系平均所需时间分别为28.8d,39.7d,46.3d.结论:CysA法成功率高,建系所需时间短,是建立B-LCL最可靠的选择.

AIM： To investigate the optimal method of establishing immortal lymphoblastoid cell bank of keloid pedigree and provide a long-term specimen resource for keloid research. METHODS： With three methods including CysA, small amount of fresh whole blood and frozen whole blood, B-lymphoblastoid cell lines （B-LCL） were established and their success rates and time were compared. RESULTS： The immortal lymphoblastoid cell lines of a keloid pedigree were obtained successfully. The success rates of transformation by CysA, small amount of fresh whole blood and frozen whole blood were 96.4%, 46.4%, and 28.6%, and the time of the three mothods were 28.8 d, 39.7 d, and 46.3 d, respectively. CONCLUSION： Among the three methods, the success rate of CysA method is highest, and the time for establishing the cell lines is shortest. So it is a reliable method to transform human B-LCL by EB virus.