山香圆总黄酮体外对大鼠佐剂性关节炎免疫功能的影响

Effect of total flavonoids of turpinia arguta seen on immune function in adjuvant arthritis rats in vitro

目的观察山香圆总黄酮（total flavonoids of turpinia arguta seen，TFS）体外对佐剂性关节炎（adjuvant arthritis，AA）大鼠免疫功能的影响。方法选择健康♂SD大鼠，采用弗氏完全佐剂（freunds complete adjuvant，FCA）诱导AA大鼠模型：将同一批号的的卡介苗80℃水浴1h灭活，用灭菌液体石蜡配成10g·L^-1的乳剂。d28处死大鼠，取AA大鼠的免疫细胞（脾细胞和腹腔巨噬细胞）体外给药培养并检测一些免疫指标：细胞增殖法检测ConA、LPS诱导的大鼠脾淋巴细胞增殖反应及IL-1、IL-2的分泌水平；放免法检测PGE2水平。结果与正常组相比，AA大鼠ConA、LPS诱导的脾淋巴细胞增殖功能低下，脾淋巴细胞产生的IL-2活性下降，LPS诱导的大鼠腹腔巨噬细胞产生的IL-1、PGE2水平明显升高。TFS（10^-8～10^-4）mg·L^-1可纠正AA大鼠低下的脾淋巴细胞增殖反应和脾细胞IL-2的产生，降低AA大鼠腹腔巨噬细胞产生过高的IL-1和PGE2。结论山香圆总黄酮对AA大鼠的异常免疫功能具有调节作用。

Aim To study the effect of the total flavonoids of turpinia arguta seen （ TFS ） on immune function of rats with adjuvant arthritis. Methods Adjuvant arthritis （AA） was induced on d0 by intradermal injection of Complete Freund＇s Adjuvant （FCA）,containing 10 mg heat-inactive BCG in 1ml paraffin oil. On d 28 after immunization, AA rats were killed by cervical decollation. Immune cells （splenocytes and peritoneal macrophages） were obtained, then cultured in vitro with TFS. Some immune indexes were detected respectively such as the splenocytes proliferation and the productions of IL-1 and IL-2 were measured by the method of cell proliferation, the production of prostaglandin E2 was determined by radioimmunoassay. Resuits Compared with normal groups, the response of splenocytes to ConA and LPS was lowered, IL-2 synthesis was decreased, and IL-1 and PGE2 released from PMΦ were elevated. TFS （10^-8～10^-4 mg·L^-1） not only increased splenocytes proliferation, which induced by ConA and LPS, and IL-2 production of splenocytes,but also reduced the elevated productions of IL-1 and PGE2 released from peritoned macrophate in AA rats in vitro. Concuision TFS could adjust abnormal immune function in AA rats.