浊度/分光光度法与毛细管等电点聚焦法测定明胶等电点及其分布的比较研究(英文)

Comparative study of determining IEP and IEP distribution of gelatin with turbidimetry/spectrometry and capillary IEP focusing methods

等电点是蛋白质最重要的物理化学性质之一.目前有多种测定等电点的方法.本文利用最简单的浊度/分光光度法和最先进的毛细管等电点聚焦法分别测定了河南牛皮明胶的等电点,对两种方法进行比较并论证了后一种方法的优势.

The isoeleetrie point （IEP） is one of the most important physieochemical properties of proteins. There are many different methods for the determination of IEP. In this paper, a comparative study between the simplest turbidimetry/speetrometry method and the most advanced capillary IEP focusing（CalPF） method was made. Henan cattle hide gelatin＇ s IEP was determined with the two methods and the advantages of the CaIPF method over the other methods were demonstrated and discussed.