番木瓜雄性性别的RAPD和SCAR标记

RAPD and SCAR molecular markers for male trait in Caric papaya

利用RAPD标记技术鉴定番木瓜雄性性状。在214条随机引物中,用引物Z18扩增得到1条雄性多态性片段,此片段存在于试验所用材料的所有雄株中而两性株和雌株没有,将其命名为Z18-1000。根据对该片段的序列分析结果重新设计了2对引物将RAPD标记成功转化成了SCAR标记,并命名为SD-1000。

The random amplified polymorphic DNA （RAPD） technique was used to determine the male trait of Caricapapaya L.. 214 10-mer primers were tested. A male-associated fragment was generated with Z18 primer. The marker fragment, named Z18-1000 existed in all male plants but not in the femaleand hermaphrodite plants so far analyzed. The fragment was cloned and sequenced. Four primers were designed based on the sequence to transform RAPD marker into SCAR marker. The RAPD marker was successfully converted into a SCAR marker, which was designated SD1000.